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. 2020 Feb 7;21(3):e49129. doi: 10.15252/embr.201949129

Figure EV3. shRNA knockdown of IGF1R in PMN.

Figure EV3

  1. Western blot of pIGF1R after lentiviral delivery of a shRNA targeting IGF1R or scrambled control in PMN. Cells were lysed 6 days after treatment.
  2. The quantification of the Western blots in A). The shRNA viral construct effectively decreases levels of pIGF1R compared to scrambled control (N = 1 independent experiments).
  3. Graph shows the average velocity of HcT‐containing organelles per axon after IGF1R knockdown (**P = 0.001, Student's t‐test, N = 3 independent experiments; boxplot shows median, first and third quartiles. Upper/lower whiskers extend to 1.5 * the interquartile range).
  4. Graph shows the average velocity of HcT‐containing organelles per experiment after IGF1R knockdown (P = 0.26, Student's t‐test, N = 3 independent experiments; boxplot shows median, first and third quartiles. Upper/lower whiskers extend to 1.5 * the interquartile range).