Skip to main content
. 2020 Jan 9;21(3):e48512. doi: 10.15252/embr.201948512

Figure EV1. Related to Fig 1. Analysis of the brain morphology of crmp2 −/− mice.

Figure EV1

  1. CRMP2 (A + B isoforms, arrows) and CRMP2A (arrowhead) isoform immunostaining of coronal brain sections from adult WT and crmp2 −/− mice. Both isoforms are missing in crmp2 −/− mice. Yellow rectangles depict the area shown in Fig 1B. CRMP2B is present throughout the cortex, and hippocampal CA1 and CA3 regions. CRMP2A is localized mostly in callosal axons (CC), mossy fibers (mf), and inner molecular layer (mo) of dentate gyrus. Scale bars: 100 μm.
  2. Western blot analysis of brain lysates of crmp2 +/+, +/− and −/− mice.
  3. Upper view of WT and crmp2 −/− brains showing similar brain size. Scale bars: 5 mm.
  4. Three 250‐μm coronal sections from n = 3 WT and crmp2 −/− mice demonstrate enlarged ventricles in crmp2 −/− mice. Scale bars: 5 mm.
  5. Quantification of the ventricle areas normalized to total slice area (ventricle/slice index, WT 0.011 ± 0.001, crmp2 −/− 0.027 ± 0.003, P < 0.001, n = 3 animals/genotype). Mean ± SD, ***P < 0.001, t‐test.
  6. Analysis of cortical neuron migration (WT/crmp2 +/− n = 6 embryos, crmp2 −/− n = 4 embryos). Both WTs and heterozygous (Het) embryos were used as controls. Embryos were electroporated with GFP expressing plasmid at E14.5 and analyzed at E17.5. Quantification of percentages of GFP‐positive neurons in different layers is shown. CP—cortical plate, IVZ—interventricular zone, VZ—ventricular zone. Scale bars: 100 μm, mean ± SD, t‐test.