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. 2020 Jan 9;21(3):e48512. doi: 10.15252/embr.201948512

Figure 3. Infrapyramidal bundle fails to prune in crmp2 −/− mice.

Figure 3

  • A, B
    (A) Coronal sections of P14 brains stained for calbindin and VgluT1. Infrapyramidal bundle (IPB) progresses into hippocampal CA3 region in both WT and crmp2 −/− mice. Nuclei are counterstained with Hoechst 33342. (B) Details from depicted regions in (A), arrowheads show the course of IPB. Scale bars: 100 μm.
  • C, D
    (C) Staining for calbindin shows unpruned infrapyramidal bundle in adult crmp2 −/− mice. No VGluT2 signal in hippocampal CA3 region is present in WT and crmp2 −/− suggesting mature synapses. Scale bars: 500 μm. (D) Details from depicted regions in (C), arrowheads show the course of IPB. Scale bars: 100 μm.
  • E, F
    Staining for calbindin and VgluT1 shows mature synapses in the main bundle in both genotypes and in the IPB in crmp2 −/− mice (arrowheads). Scale bars: 100 μm.
  • G
    Quantification of IPB index (length of the IPB vs. main bundle, WT 0.43 ± 0.05, crmp2 −/− 0.78 ± 0.05, P < 0.001) counted from adult coronal sections (WT n = 4 mice, crmp2 −/− n = 5 mice). Mean ± SD, ***P < 0.001, t‐test.