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. 2020 Jan 9;21(3):e48512. doi: 10.15252/embr.201948512

Figure 5. CRMP2 mediates Sema3F signaling in primary neurons.

Figure 5

  1. Time‐lapse imaging of DIV7 cultured hippocampal neurons before and after semaphorin stimulation. Upper panel: axon growth without semaphorin stimulation. Middle panel: stimulation with Sema3A (0 h) (1 nM, n = 669 axons for WT, 761 axons for knockout) causes retraction of both WT and crmp2 −/− neurons. Lower panel: stimulation with Sema3F (5 nM, n = 602 axons for WT, 955 axons for knockout) causes axon retraction in WT, but not in crmp2 −/− neurons. Red triangles depict growing axons, yellow retracting axons, and blue asterisks indicate steady non‐growing axons. See also [Link], [Link]. Scale bars: 100 μm (whole image field) and 50 μm (magnified).
  2. Schematic drawing of the experimental setup.
  3. Quantification of retracting axons (number of retracting vs. steady axons, three experiments). WT: control 13.4 ± 5%, Sema3A 31.2 ± 6% (P < 0.001), Sema3F 36.9 ± 8.7% (P < 0.001); crmp2 −/−: control 19.8 ± 1.8%, Sema3A 28.5 ± 4.4% (P < 0.05), Sema3F 22.4 ± 4.1% (P > 0.99), mean ± SD are shown. ***P < 0.001, *P < 0.05, 2‐way ANOVA with Bonferroni's multiple comparison test.