Skip to main content
. 2020 Jan 9;21(3):e48512. doi: 10.15252/embr.201948512

Figure 6. CRMP2 regulates dendritic spine refinement in dentate gyrus granule cells.

Figure 6

  • A, B
    Dendritic spine density in DiOlistically labeled DG granule cells is similar in WT and crmp2 −/− mice at P30. In adults, however, spine density in crmp2 −/− granule cells is increased comparing to WT. Scale bars: 5 μm.
  • C
    Quantification of dendritic spine density in DG granule cells in the inner molecular layer (50–100 μm away from the soma). WT, P30: 12.5 ± 2.2 spines/10 μm, adults: 9.95 ± 2.3; crmp2 −/−, P30: 12.86 ± 2.1 (P > 0.99), adults: 12.88 ± 2.9 (P < 0.001), mean ± SD, ***P < 0.001, 2‐way ANOVA with Bonferroni correction. P30: 3 animals/genotype, WT = 61 dendrites, knockout = 64 dendrites; adults: 3 animals/genotype, WT = 41 dendrites, knockout = 37 dendrites.
  • D, E
    Analysis of branching of DiOlistically labeled DG granule cell dendrites in adult WT and crmp2 −/− mice (n = 3 animals, ≥ 25 dendrites). Quantification of granule cell branching by Sholl analysis showed no significant differences (P > 0.99). Scale bars: 50 μm, mean ± SEM, 2‐way ANOVA with Bonferroni correction.
  • F
    Defects in synapse elimination in the inner molecular layer revealed by double immunostaining with PSD95 and VGluT2 antibodies (n = 5 mice/genotype). GL indicates granule cell layer, and IML/OML indicate inner/outer molecular layer, respectively. Density of colocalized PSD95/VGLuT2 puncta was counted. IML: WT 109 ± 21, crmp2 −/− 156 ± 15 (P = 0.004). OML: WT 281 ± 38, crmp2 −/− 297 ± 80 (P = 0.7). Scale bars: 10 μm, mean ± SD, *P < 0.05, t‐test.
  • G
    Spine density in DiOlistically labeled prefrontal cortex (PFC) is similar in WT and crmp2 −/− mice in both juvenile and adult mice. Scale bars: 5 μm.
  • H
    Quantification of dendritic spine density of PFC pyramidal neurons, basal dendrites (WT, juvenile: 8.63 ± 1.44 spines/10 μm, adults: 7.3 ± 1.4; crmp2 −/−, juvenile: 9.05 ± 1.73, P = 0.97, adults: 6.98 ± 1.53, P > 0.99), mean ± SD, 2‐way ANOVA with Bonferroni's multiple comparison test. P25: n = 3, ≥ 50 dendrites; adults: n = 3, ≥ 50 dendrites.