Figure EV1. Mobility analysis of structures positive for p62 and/or NBR1.

• A
  Wild‐type hepatocytes were infected with GFP or NBR1‐GFP adenovirus for 48 h and then immunostained with anti‐p62 antibody. Each inset is a magnified image. Bars: 20 μm.
• B–D
  FRAP assay. p62‐knockout MEFs were co‐transfected mCherry‐p62 together with GFP, GFP‐NBR1, or GFP‐NBR1 D50R. Forty‐eight hours after the transfection, the signal recovery after photobleaching was measured and quantified (B). GFP‐NBR1 was transfected into wild‐type or p62‐knockout MEFs. Forty‐eight hours after the transfection, the signal recovery after photobleaching was measured and quantified (C). GFP‐p62 was transfected into wild‐type or Nbr1‐knockout MEFs. Forty‐eight hours after the transfection, the signal recovery after photobleaching was measured and quantified (D). Three independent biological replicates were performed on each experiment. Data are shown as means ± SE. *P < 0.05 as determined by Welch's t‐test.