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. 2020 Jan 9;21(3):e48902. doi: 10.15252/embr.201948902

Figure EV2. NBR1 influences Nrf2 activation but not other p62‐related pathways.

Figure EV2

  1. The primary hepatocytes prepared from wild‐type mice and infected with adenovirus GFP or NBR1 for 48 h employed in Fig 1A were subjected to immunoblot analysis with the indicated antibodies. Data shown are representative of three separate experiments. Bar graphs indicate the quantitative densitometric analysis of the indicated proteins relative to actin. Data are shown as means ± SE. *< 0.05 as determined by Welch's t‐test.
  2. Primary hepatocytes prepared from p62 f/f; Alb‐Cre mice were infected with adenovirus expressing GFP or NBR1 in combination with p62 for 48 h. Cell lysates were prepared and subjected to immunoblot analysis with the indicated antibodies. Data shown are representative of three separate experiments. Bar graphs indicate the quantitative densitometric analysis of p62 relative to actin. Data are shown as means ± SE. *< 0.05 as determined by Welch's t‐test.

Source data are available online for this figure.