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. 2020 Jan 13;21(3):e48328. doi: 10.15252/embr.201948328

Figure EV1. Overexpressing Tau increases β‐catenin protein level without affecting the mRNA level of β‐catenin.

Figure EV1

  • A, B
    Overexpressing Tau did not affect mRNA level of β‐catenin. HEK293 (A) and N2a (B) cells were transfected respectively with Tau40 (Tau) or the empty vector (Vec) for 48 h. β‐catenin mRNA level (ctnnb1) was detected by RT–PCR (n = 6–9 biological replicates each group).
  • C–H
    Overexpressing Tau increased β‐cat protein level in N2a cells (transfected with Tau40 or Vec for 48 h; C, D), cultured hippocampal neurons (cultured with 7 div and then transfected with AAV‐eGFP‐vector or AAV‐eGFP‐Tau for 5 div; E, F), and in the hippocampi of 10‐month‐old Tau transgenic mice (Tau+) (G, H) measured by Western blotting (n = at least three biological replicates each group).
  • I
    The protein level of β‐cat protein (upper panels) was significantly increased in hippocampal cornu ammonis 1(CA1), cornu ammonis 3 (CA3), and dentate gyrus (DG) subsets of 10‐month‐old Tau transgenic mice (Tau+) compared with Tau knockout mice (Tau−), with significantly increased total Tau (HT7, middle panels) and the phosphorylated Tau (AT8, lower panels). Scale bar, 50 μm.
Data information: Data are presented as mean ± SEM; **< 0.01; ***< 0.001 vs. Vec (A, B, D, and F) or vs. Tau− (H). Data were analyzed by Student's t‐test.Source data are available online for this figure.