Table 2.
Humoral and Cellular Immune Responses to AAV Capsid
| Route and Animal No. | Anti-AAV1 IgG Concentration (μg/mL) |
Anti-AAV1 NAb Titer |
IFNγ Secretion to rAAV1 Capsid | ||||
|---|---|---|---|---|---|---|---|
| Pre-dose | Day 21 | Day 60 | Pre-dose | Day 21 | Day 60 | ||
| IM | |||||||
| RA1598 | ND | 18.2 | 76.4 | <1:10 | 1:640 | 1:1,280 | − |
| RA1683 | 0.6 | NA | 1,080.2 | 1:10 | 1:1,280 | 1:20,480 | − |
| RA0764 | ND | 30.0 | 530.1 | <1:10 | 1:1,280 | 1:10,240 | − |
| VLP | |||||||
| RA1567 | ND | 91.2 | 139.7 | <1:10 | 1:2,560 | 1:5,120 | − |
| RA0770 | ND | 55.4 | 170.4 | <1:10 | 1:1,280 | 1:5,120 | − |
| RA0332 | ND | 51.3 | 159.7 | 1:10 | 1:1,280 | 1:5,120 | + |
| IAPD | |||||||
| RA1562 | ND | 19.8 | 120.4 | <1:10 | 1:160 | 1:1,280 | − |
| RA1709 | 0.3 | 32.0 | 241.3 | <1:10 | 1:320 | 1:1,280 | − |
IM, intramuscular; VLP, venous limb perfusion; IAPD, intra-arterial push and dwell; NAb, neutralizing antibodies; ND, not detected, NA, not available; −, no response; +, positive response.