Skip to main content
. 2020 Jan 10;28(3):794–804. doi: 10.1016/j.ymthe.2020.01.003

Figure 1.

Figure 1

Cytotoxic Activity of OBP-502 against Murine Gastrointestinal Cancer Cell Lines

(A) Viability of CT26 and PAN02 cells was assessed using an XTT assay 3 days after OBP-502 treatment at the indicated doses (MOI). The percentage of viable cells relative to non-treated cells (0 MOI) was plotted. Error bars indicate 95% confidence intervals. (B) Whole-cell lysates of CT26 and PAN02 cells collected 3 days after OBP-502 treatment (0, 10, 100, 500, and 1,000 MOI) were subjected to western blot analysis of E1A, PARP, p62, PD-L1, and β-Actin expression. Cleaved PARP (C-PARP) and p62 indicate induction of apoptosis and autophagy, respectively. (C) CT26 and PAN02 cells treated with OBP-502 (1,000 MOI) were subjected to flow cytometry for analysis of PD-L1 expression on the cell surface 3 days after treatment.