Table 1.

Effect of Apigenin Treatment on OVA-Induced Alterations in Body Weight, Relative Spleen Weight, OVA Challenge-Induced Nasal Rubbing, Sneezing, and Nasal Discharge as Well as Histamine Challenge-Induced Nasal Rubbing and Sneezing in AR Mice.^a

Parameters	Treatment
	Normal	AR Control	MLT (10)	AP (5)	AP (10)	AP (20)	Per se
Body weight (g) on day 21	31.17 ± 1.25	23.50 ± 1.06b	29.83 ± 1.54c,d	26.50 ± 1.82	28.00 ± 1.21c,d	29.67 ± 0.71c,d	30.33 ± 1.31
Spleen wt/body wt (mg/g) (×10−3) on day 21	3.37 ± 0.13	6.68 ± 0.35b	3.92 ± 0.23c,d	5.96 ± 0.35	4.80 ± 0.34c,d	3.73 ± 0.09c,d	3.34 ± 0.29
OVA challenge on day 21
Rubbing (number)	15.17 ± 2.3	71.33 ± 2.16b	23.67 ± 1.33c,d	66.17 ± 2.04	48.83 ± 1.28c,d	27.00 ± 1.63c,d	15.83 ± 1.38
Sneezing (number)	10.83 ± 1.08	41.33 ± 0.76b	13.17 ± 0.95c,d	35.17 ± 1.42	28.67 ± 0.80c,d	18.50 ± 0.99c,d	12.50 ± 0.99
Discharge (score)	0.33 ± 0.21	2.50 ± 0.22b	0.50 ± 0.22c,d	2.33 ± 0.21	1.33 ± 0.21c,d	0.50 ± 0.22c,d	0.33 ± 0.21
Histamine challenge on day 24
Rubbing (number)	17.83 ± 3.33	71.5 ± 2.59b	24.00 ± 2.53c,d	66.83 ± 2.68	44.33 ± 1.65c,d	32.33 ± 3.65c,d	21.00 ± 3.16
Sneezing (number)	8.67 ± 2.08	52.33 ± 2.45b	16.00 ± 1.55c,d	50.33 ± 1.43	31.00 ± 3.13c,d	21.33 ± 2.53c,d	10.67 ± 1.12

Abbreviations: ANOVA, analysis of variance; AP (5), apigenin (5 mg/kg) treated; AP (10), apigenin (10 mg/kg) treated; AP (20), apigenin (20 mg/kg) treated; AR, allergic rhinitis; MLT (10), montelukast (10 mg/kg) treated; OVA, ovalbumin; SEM, standard error of the mean.

^a Data are represented as mean ± SEM (n = 4-6). Data for body weight and relative spleen weight were analyzed by one-way ANOVA followed by Tukey multiple range test, whereas data of OVA and histamine challenge number and score were analyzed by nonparametric Kruskal-Wallis test ANOVA followed by Mann-Whitney’s multiple comparison tests. Figures in parentheses indicate a dose in mg/kg.

^b P < .05 as compared with normal group.

^c P < .05 as compared with AR control group.

^d P < .05 as compared montelukast with apigenin.