Figure 7.

The circCAMSAP1/miR-328-5p/E2F1 Axis Is Essential for CRC Progress In Vivo

The sh1-mediated circCAMSAP1 silencing HCT15 cells were treated with antagomir-control or antagomir-328-5p. (A) Images of tumors of indicated groups (n = 8). (B) Tumor growth curves of nude mice with indicated treatments. Tumor volumes were monitored with digital calipers every other day during the time course of 4 weeks. (C) Tumor weights were measured at day 28. (D) H&E staining and IHC staining of Ki-67, E2F1, and Cyclin E in tumors with indicated treatments. Scare bars, 100 μm. Original magnification, ×200. ***p < 0.001 by Student’s t test. Error bars indicate SEM.