Table 2.
PCR condition.
| Thermal cycler | Initial steps | Times and temperatures | ||
|---|---|---|---|---|
| Each of 45 cycles | ||||
| Melt | Annealed | Extended | ||
| PCR | HOLD | CYCLE | ||
| 3 min 95°C | 7 s 95°C | 10 s 57°C | 15 s 72°C | |
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