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Effect of KXS on NEP activity in PC12 cells. PC12 cells were treated with 10% rat serum containing KXS in the culture medium, thiorphan (NEP inhibitor, 10 μM), or KXS plus thiorphan for 24 h, respectively. Then PC12 cells were incubated with Aβ (1 μM) for 3 h in the culture medium and extracellular Aβ concentration was investigated using the ELISA kit. Data were expressed as mean ± S.D (∗∗P < 0.01 vs. control; n = 3).
