Figure 2.

Purified IgG Enhances Ad5 Transduction of DCs

(A) A protein G column was used to purify IgG from serum N50, and the different fractions were used to probe a western blot of denatured Ad5 vector particles. Following transfer, narrow strips of the PVDF membrane were cut and incubated with serum N50 (Pre), unbound flowthrough (F), bound eluate (E), and rabbit antiserum (R). The migration of protein standards, along with their respective molecular weights, is shown in the right lane (M). The Ad5 capsid proteins hexon (H), penton (P), and fiber (F) are indicated by arrows. (B) Serum N50 fractions were further analyzed for enhanced transduction of RAW264.7 cells, a murine macrophage cell line, by incubating them with Ad5-GFP before being added to cells. Panels show GFP expression in cells at 48 h following pre-incubation of vector (MOI, 10⁴) with different fractions.