Figure 4.

Activation of Murine DCs and Enhanced In Vivo Inflammatory Responses to Ad5 Immune Complexes

(A) Bone marrow DCs (10⁶) from C57BL/6 mice were transduced with Ad5-GFP (MOI, 10⁴) particles, Ad5 pre-complexed with rabbit antiserum, or Ad5-pre-complexed with IV-Ig. Cells were imaged 48 h later using an inverted fluorescent microscope, and GFP and CD80 expression were quantified using flow cytometry. IL-6 release in supernatants was determined using a mouse Luminex multi-analyte assay. (B) Ad5-GFP (10¹¹ vp) was injected intravenously into the tail vein of C57BL/6 mice that previously received IV-Ig. IL-6 (pg/mL) is plotted against the expected final IV-Ig concentration (mg/mL). We used a t test to compare IL-6 levels at a given final IV-Ig concentration with and without Ad5 vector. *p < 0.05. (C) Serum IL-6 levels were measured 6 and 72 h after vector administration in animals that received IV-Ig + Ad5 or IV-Ig alone. (B and C) Each data point represents IL-6 levels in three mice (mean ± SD) for IV-Ig alone (circles) and IV-Ig + Ad5 (squares).