Figure 3.

Sirt1 Has No Effect on Chemokine Secretion and Chemotactic Activity of Inflammatory Cytokine-Induced MSCs

(A) The expression of chemokine genes Cxcl9, Cxcl10, Cxcl11, Icam1, and Vcam1 was quantified by real-time PCR in MSCs, AdEGFP-MSCs, and AdSirt1-MSCs with or without stimulation of IFN-γ (10 ng/mL) and TNF-α (10 ng/mL) for 8 h. ***p < 0.001 versus MSC; p > 0.05 versus IT-MSC; NS, not significant (p > 0.05). (B) MSCs, AdEGFP-MSCs, or AdSirt1-MSCs pretreated with IFN-γ and TNF-α (10 ng/mL each) for 24 h were respectively seeded in the lower chamber, and splenocytes were added to the upper chamber. After 0 or 3 h of incubation, the extent of cell aggregation was screened under light microscopic observation. (C) Quantification of splenocyte aggregation was measured by counting the total number of migrated splenocytes in each group. p > 0.05 versus IT-MSC; NS, not significant (p > 0.05).