KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and Virus Strains
BL21(DE3) E. Coli	Thermo Fisher	Cat#: EC0114
Chemicals, Peptides, and Recombinant Proteins
Human: SUMO	Boston Biochem	Cat #: UL-712
Human: His6-SENP1 catalytic domain	Boston Biochem	Cat#: E-700
Ranolazine dihydrochloride	Sigma-Aldrich	Cat#: R6152
Experimental Models: Cell Lines
Hamster: CHO-K1 cells	ATCC	Cat#: CCL-61; RRID: CVCL_0214
Human: iPS-ventricular cardiomyocytes-2	Cellular Dynamics	Cat# CMC-100-012-000.5
Recombinant DNA
hNaV1.5-pcDNA1	Plant et al., 2006	DOI: 10.1172/JCI25618
GFP-SCN1B-pcDNA1	Plant et al., 2006	DOI: 10.1172/JCI25618
mCherry-SUMO1-pMAX	Plant et al., 2016	https://doi.org/10.7554/eLife.20054
mTFP1-hNav1.5-pMAX	This paper	N/A
eYFP-SUMO1-pMAX	Plant et al., 2010	https://doi.org/10.1073/pnas.1004712107
eYFP-Ubc9-pMAX	Plant et al., 2010	https://doi.org/10.1073/pnas.1004712107
Plasmid for MS with SUMO1(T95K)1–97	Plant et al., 2010	https://doi.org/10.1073/pnas.1004712107
Plasmid for MS with Human NaV1.5 residues 353–502	Plant et al., 2010, modified as described	https://doi.org/10.1073/pnas.1004712107
Software and Algorithms
pClamp version 10	Molecular Devices	N/A
Origin version 6	Microcal	N/A
Prism version 8	GraphPad	N/A
ImageJ-FIJI	Schindelin et al., 2012; https://imagej.net/Fiji	https://doi.org/10.1038/nmeth.2019
MSConvert	http://proteowizard.sourceforge.net/tools.shtml
Batch-Tag in Protein Prospector v. 6.1.0	http://prospector.ucsf.edu/
O’Hara Rudy Model (ORD)	O’Hara et al., 2011	https://doi.org/10.1371/journal.pcbi.1002061