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. 2020 Feb 3;152(3):e201912471. doi: 10.1085/jgp.201912471

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© 2020 Klipp et al.

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Figure 5. — ASIC2a does not directly interact with STOM. (A) Top: Representative current traces of ASIC2a elicited by a switch to pH 4.0 with and without coexpression of STOM. Bottom: Average current densities for ASIC2a in the absence (black) and presence (red) of STOM were 95.9 ± 20.7 pA/pF (n = 14) and 101.9 ± 14.9 pA/pF (n = 14), respectively. (B) Plot showing the fraction of ASIC2a current remaining at 2 and 6 s. (C) Plot of the FRET efficiency between ASIC2a-CER and STOM-YFP. Average FRET efficiency of ASIC2a-CER + STOM-YFP = 1.0 ± 0.02% (n = 8). Dotted line in FRET plots corresponds to control WT ASIC3-CER/STOM-YFP FRET signal replotted from Fig. 1 E for comparison. Data are given as mean ± SEM.