Table 1. Biophysical properties of NaV1.2 variants.
Variant | Voltage dependence of activation | Voltage dependence of fast inactivation | Recovery from fast inactivation (−10 mV) | ||||||
---|---|---|---|---|---|---|---|---|---|
V1/2 (mV) | k (mV) | n | V1/2 (mV) | k (mV) | n | τf (ms) (amplitude) | τs (ms) (amplitude) | n | |
NaV1.2A | −23.5 ± 1.3 | 6.3 ± 0.3 | 15 | −63.5 ± 0.9 | −6.2 ± 0.1 | 15 | 1.9 ± 0.1 (0.90 ± 0.01) | 275.6 ± 16.4 (0.12 ± 0.01) | 15 |
NaV1.2A-T236S | −25.6 ± 2.0 | 7.0 ± 0.3 | 9 | −65.3 ± 1.8 | −5.6 ± 0.1 | 9 | 2.1 ± 0.1 (0.87 ± 0.01) | 270.1 ± 16.8 (0.14 ± 0.01) | 8 |
NaV1.2A-E999K | −4.6 ± 1.4 | 6.5 ± 0.3 | 10 | −63.1 ± 1.2 | −6.2 ± 0.1 | 10 | 1.9 ± 0.2 (0.90 ± 0.01) | 269.3 ± 21.2 (0.14 ± 0.01) | 10 |
NaV1.2A-S1336Y | −23.2 ± 1.4 | 7.9 ± 0.4 | 11 | −57.7 ± 1.3a | −6.6 ± 0.3 | 11 | 1.3 ± 0.2 (0.84 ± 0.03) | 210.3 ± 20.4 (0.21 ± 0.02) | 11 |
NaV1.2A-T1623N | −18.4 ± 1.4a | 6.4 ± 0.6 | 8 | −56.7 ± 0.8a | −6.0 ± 0.1 | 8 | 2.1 ± 0.1 (0.92 ± 0.01) | 240.4 ± 17.4 (0.09 ± 0.01) | 8 |
NaV1.2A-R1882Q | −19.5 ± 0.9 | 6.7 ± 0.3 | 15 | −51.2 ± 1.5a | −7.6 ± 0.3a | 15 | 1.6 ± 0.1 (0.84 ± 0.02) | 248.2 ± 22.0 (0.16 ± 0.02) | 15 |
NaV1.2N | −19.5 ± 0.8b | 7.0 ± 0.3 | 15 | −62.4 ± 1.2 | −6.4 ± 0.1 | 15 | 1.7± 0.1 (0.90 ± 0.01) | 267.3 ± 28.8 (0.13 ± 0.01) | 15 |
NaV1.2N-T236S | −29.9 ± 1.1a | 5.7 ± 0.4a | 15 | −62.3 ± 0.8 | 6.1 ± 0.4 | 8 | 1.9 ± 0.1 (0.87 ± 0.01) | 245.3 ± 7.7 (0.14 ± 0.01) | 7 |
NaV1.2N-E999K | −26.2 ± 1.7a | 6.3 ± 0.4 | 14 | −63.1 ± 1.3 | −5.8 ± 0.1 | 13 | 1.8 ± 0.1 (0.89 ± 0.01) | 263.2 ± 22.9 (0.12 ± 0.01) | 13 |
NaV1.2N-S1336Y | −25.3 ± 1.9a | 7.3 ± 0.7 | 10 | −56.9 ± 1.0a | −7.9 ± 0.8a | 10 | 1.0 ± 0.1 (0.89 ± 0.02) | 261.0 ± 38.8 (0.16 ± 0.03) | 10 |
NaV1.2N-T1623N | −18.4 ± 1.4 | 6.2 ± 0.3 | 9 | −56.3 ± 0.7a | −6.3 ± 0.4 | 9 | 2.0 ± 0.1 (0.87 ± 0.02) | 213.6 ± 23.9 (0.14 ± 0.02) | 9 |
NaV1.2N-R1882Q | −20.6 ± 1.7 | 6.2 ± 0.4 | 14 | −53.4 ± 1.3a | −7.0 ± 0.2a | 14 | 1.6 ± 0.1 (0.88 ± 0.02) | 258.2 ± 27.8 (0.12 ± 0.02) | 14 |
P < 0.05 between WT and variant in same splice isoform.
P < 0.05 between neonatal and adult isoforms.