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. 2020 Feb 26;11:98. doi: 10.3389/fphar.2020.00098

Figure 1.

Figure 1

EGF induces the migration of OSCC cells. The results of the wound healing assay and quantification of migration rate (A), showing that the cell migration was increased by EGF stimulation for 24 h. *P < 0.05, **P < 0.01. Scale bar, 100 μm. (B) The results of the CCK8 assay showed that treatment with the indicated concentration of EGF for 24 h did not noticeably affect the viability of CAL27 cells. (C) qPCR detection of WNT mRNA expression in CAL27 cells showed that WNT4, WNT7A, WNT7B, and WNT10A were abundantly expressed. (D) qPCR detection of WNT mRNA expression in HSC3 cells. (E) Among the highly expressed WNT mRNAs, the WNT7A mRNA level was doubled with EGF (20 ng/mL) treatment for 24 h. *P < 0.05, **P < 0.01. (F) The qPCR result, which showed the maximum change in MMP mRNA expression with EGF (20 ng/mL) stimulation for 24 h. **P < 0.01. (G) The results of Western blotting showed that WNT7A was remarkably increased after EGF (20 ng/mL) stimulation in both HSC3 and CAL27 cells. (H) The results of CCK8 assay in CAL27 and HSC3 cells showed that there was no significant difference between the two indicated groups at 0, 12, 24, and 48 h. (I) EDU assay showed that EGF (20 ng/mL) treatment for 24 h did not affect cell proliferation in CAL27 and HSC3 cells.