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. 2020 Feb 26;11:98. doi: 10.3389/fphar.2020.00098

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Copyright © 2020 Xie, Ma, Li, Chen, Xie, Chen, Zhao, Tang, Zhao, Zhang, Du, Zhang and Gu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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EGF regulates the activation of the WNT7A/β-catenin pathway. (A, B) CAL27 cells were incubated with EGF (20 ng/mL) for the indicated times. The results of Western blotting showed that the nuclear distribution of β-catenin increased and reached the peak level at 8-12 h with EGF treatment. ^* P < 0.05 the cytoplasm versus the nucleus. (C) CAL27 and HSC3 cells were divided into the indicated groups for immunofluorescent microscopy. The results showed that β-catenin accumulated in the nucleus with EGF stimulation as well as WNT7A overexpression. Knocking down WNT7A blocked the entrance of β-catenin into the nucleus in response to EGF stimulation. Scale bar, 10 μm. (D) In CAL27 cells incubated with 20 ng/mL EGF, the protein level of β-catenin in the nucleus was decreased by WNT7A silence. (E) β-catenin accumulated in the nuclei of CAL27 cells overexpressing WNT7A.