Figure 6.

Decrease of mobile Zn levels in atg mutant plants causes decay of Zn-requiring enzyme activity. A, Proteins were extracted from Zin-Pro Capture (ZPC)-treated plants (NahG and NahG atg5, 3 DAT to −Zn), and subjected to SDS-PAGE; fluorescence was detected in-gel. The lower representation shows a CBB-stained gel, confirming equal protein loading. Approximate molecular mass (kilodaltons) is shown on the right. B, SOD activities of NahG and NahG atg5 plants at 5 DAT to −/+Zn. Gray parts indicate Cu/ZnSOD activities, and white parts indicate MnSOD and FeSOD activities. n = 4. Error bars show SD.