Figure 2.

Transcription of wheat NRT2 and NAR genes in different organs at different developmental stages. A to F, Transcription of wheat NRT2 genes. G to I, Transcription of wheat NAR genes. According to the classic Zadoks scale (Zadoks et al., 1974), transcript libraries of different tissues were obtained at Z11 (seedling stage, column in orange), Z21 (tillering stage, column in green), Z32 (jointing stage, column in blue), and Z59 (flowering period, column in dark orange), respectively. SR and SS, Respectively, root and shoot at the seedling stage; TR, TS, and TL, respectively, root, shoot, and leaf at the tillering stage; JR, JS, and JL, respectively, root, stem, and leaf at the stem elongation stage; FR, FS, FL, FN, FFL, and FF, respectively, the root, stem, leaf, node, flag leaf, and inflorescence at the flowering period; N/A, no data available. TaEF1-α (M90077; Paolacci et al., 2009) was chosen as the endogenous control. Each bar represents the mean ± sd of at least three biological replicates.