Figure 2.

Identification of protein abundance clusters in total protein fractions derived from Arabidopsis siliques, seeds, and seedlings. A, Hierarchical clustering analysis of the normalized protein abundance over each of the developmental stages examined. In total, 40 row clusters were defined. Clusters harboring >20 proteins are labeled C1–C16. B and C, The expression profiles of all proteins in Clusters 1 and 2 (B; pink and brown lines; seed-specific proteins) and Clusters 11 and 12 (C; red and blue lines; early seedling establishment proteins) are shown. As silique samples also contain proteins derived from the silique wall, they should not necessarily be considered seed precursors, as indicated by the interrupted line between the II and RS samples. I, Phase I siliques; II, phase II siliques; RS, rehydrated seeds; StS, stratified seeds; 24 to 60 h, seedlings 24 to 60 h after stratification.