Figure 4.

Enrichment analysis of proteins in the LD-enriched fractions derived from Arabidopsis siliques, seeds, and seedlings. A volcano plot was constructed to visualize proteins that are significantly LD enriched (upper right). The developmental stage (siliques, seeds, and seedlings) with the highest abundance (riBAQ) was chosen for each protein and the log₂-transformed values and P-values were calculated at this stage. Only proteins detected in four of the five replicates and with an riBAQ >0.1 are included in this figure. As depicted in the legend, known LD and peroxisomal proteins are indicated in blue and orange, respectively, and the proteins chosen for further study that did or did not localize to LDs in pollen tubes and tobacco leaves are shown in blue and green, respectively. Black lines indicate a false discovery rate of 0.001. Abbreviations: ATS3A, EMBRYO-SPECIFIC PROTEIN; BCSAP, BRISC COMPLEX SUBUNIT ABRO1-LIKE PROTEIN; CCLP, CURCULIN-LIKE LECTIN FAMILY PROTEIN; SMT, STEROL METHYLTRANSFERASE; TMPU, TRANSMEMBRANE PROTEIN OF UNKNOWN FUNCTION.