Table 3. Arabidopsis proteins chosen as LD protein candidates.
In part, proteins were named in this study. ATS3A, EMBRYO-SPECIFIC PROTEIN; BCSAP, BRISC COMPLEX SUBUNIT ABRO1-LIKE PROTEIN; CCLP, CURCULIN-LIKE LECTIN FAMILY PROTEIN; TMPU, TRANSMEMBRANE PROTEIN OF UNKNOWN FUNCTION.
| Protein Name | AGI Codea | Homolog | Descriptionb | Stage with Highest Abundance in LD Fraction | Abundance at Highest Stage (riBAQ ‰) | Enrichment Factorc | P-Value (−Log)c | Subcellular Localizationd |
|---|---|---|---|---|---|---|---|---|
| ATS3A | AT2G41475.1 | AT5G62200 | Embryo-specific protein 3 | 48 h seedlings | 1.17 ± 0.54 | 84.4 | 6.12 | Non-LD foci |
| BCSAP | AT3G08780.1 | – | BRISC complex subunit Abro1-like protein | 60 h seedlings | 0.17 ± 0.05 | 16.3 | 7.34 | Cytoplasm |
| CCLP | AT1G78830.1 | – | Curculin-like lectin family protein | 60 h seedlings | 0.36 ± 0.1 | 35.0 | 8.24 | Non-LD foci |
| LDAH1 | AT1G10740.1* | AT1G23330* | α/β-hydrolase | 60 h seedlings | 0.19 ± 0.07 | 19.1 | 6.72 | LD |
| LDDH1 | AT1G75180.1 | AT1G19400 | Dehydrogenase | 60 h seedlings | 0.26 ± 0.04 | 18.4 | 6.11 | LD |
| LDPS | AT3G19920.1 | AT5G64230 | Unknown | Stratified seeds | 0.31 ± 0.19 | 7.4 | 2.36 | LD |
| LIDL1 | AT1G18460.1 | AT1G19360 | Lipase family protein | 60 h seedlings | 0.31 ± 0.15 | 26.9 | 6.01 | LD |
| LIME1 | AT4G33110.1 | AT4G33120 | Coclaurine methyltransferase | 48 h seedlings | 1.12 ± 0.62 | 30.3 | 3.55 | LD |
| OBAP1A | AT1G05510.1 | AT5G45690 | Oil body-associated protein | Rehydrated seeds | 1.95 ± 1.19 | 0.22 | 2.65 | Cytoplasm |
| SEC61γ | AT5G50460.1* | AT4G24920* | Subunit of SEC61 translocon | Stratified seeds | 0.96 ± 1.2 | 11.9 | 1.02 | ER/LD |
| SLDP1 | AT1G65090.3 | AT5G36100 | Unknown | 60 h seedlings | 2.1 ± 0.45 | 17.5 | 6.31 | LD |
| TMPU | AT1G27290.1 | – | Transmembrane protein of unknown function | 60 h seedlings | 1.12 ± 0.47 | 97.0 | 7.36 | ER subdomains |
Gene locus identifier according to the Arabidopsis Genome Initiative. The given splice variant corresponds to the one tested for subcellular localization. Arabidopsis Genome Initiative codes marked with an asterisk were not identified unambiguously.
Description based on TAIR10 annotation.
Enrichment factors and P-values were calculated from the given developmental stages at which the protein had the highest riBAQ intensity value in the LD-enriched fraction. Missing values were imputed with a value of 0.01.
Subcellular localizations found in this study