Table 4. Posttranslational modifications detected on LD proteins and ubiquitin.
Sites marked with an asterisk were not identified unambiguously. For a complete list of modified proteins and modification sites, see Supplemental Datasets 21–30. RS, rehydrated seeds; StS, stratified seeds.
| Protein | Ubiquitination | Phosphorylation | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| RS | StS | 24-h Seedlings | 36-h Seedlings | 48-h Seedlings | 60-h Seedlings | RS | StS | 24-h Seedlings | 36-h Seedlings | 48-h Seedlings | 60-h Seedlings | |
| OLE2 | – | – | – | – | K146 | – | S18 | – | – | – | – | – |
| OLE4 | K157* | K157* | K43 | K157* | K157* | K157* | Y153 | Y153 | S40 | – | – | – |
| K159* | K159* | K157* | K159* | K159* | K159* | Y164 | – | Y42 | – | – | – | |
| K168 | – | K159* | K168 | K168 | K168 | – | – | – | – | – | – | |
| – | – | K168 | – | – | – | – | – | – | – | – | – | |
| CLO1 | – | K4 | – | – | – | – | – | – | – | – | – | – |
| HSD1 | – | – | – | K289 | – | – | S340 | S348 | – | T283 | – | – |
| – | – | – | K295 | – | – | T343 | – | – | – | – | – | |
| – | – | – | – | – | – | S348 | – | – | – | – | – | |
| LDAP2 | – | – | – | K108 | – | – | – | – | – | T118 | – | S128 |
| – | – | – | K125 | – | – | – | – | – | S122 | – | – | |
| UBQ13 | – | – | K48 | K6 | K48 | – | – | – | S246 | T7 | S246 | – |
| – | – | K63 | K11 | K63 | – | – | – | S247 | T9 | S247 | – | |
| – | – | – | K48 | – | – | – | – | – | S246 | T249 | – | |
| – | – | – | K63 | – | – | – | – | – | S247 | – | – | |