Figure 3.

Map-based cloning of ZmEHD1. A, Schematic representation of the positional cloning of the ZmEHD1 gene on chromosome 4. The SSR markers, umc1716 and umc1650, were used for rough mapping. Recombinants are indicated below each SSR marker. B, Gene structure of ZmEHD1. Black boxes indicate exons, and lines between black boxes represent introns. The positions of mutations are marked. The mutations that led to the amino acid change between the ehd1 mutant and the wild type are indicated with red letters. C, Schematic representation of the predicted structure of ZmEHD1. The regions encoding the potential protein domains are shown. The positions of mutations on the coiled-coil domain are marked by asterisks.