Figure 6.
ZmEHD1 directly interacts with the ZmAP2 σ subunit in plants and yeast. A, Interaction between ZmEHD1 and the ZmAP2 σ subunit in maize mesophyll protoplasts observed by BiFC assays. Representative photographs are shown. Scale bars = 10 μm. B, Interaction between ZmEHD1 and the ZmAP2 σ subunit as indicated by split-ubiquitin yeast two-hybrid assays. The ZmAP2 σ subunit was used as the fused bait protein (AP2 σ-Cub), and ZmEHD1 was used as the fused prey protein (NubG-ZmEHD1). The presence or absence of His or X-Gal is indicated. C, Pull-down assays for the interaction between ZmEHD1 and the ZmAP2 σ subunit. The ZmEHD1/ZmEHD1mut in the pulled-down fraction was detected by immunoblot using anti-His antibody.