Auxin distribution and ZmPIN1a-YFP localization were altered in the ehd1 mutant relative to the wild type (WT). A, The response of horizontally placed mesocotyl-coleoptiles to gravity is delayed in the ehd1 mutant. B, Characterization of the gravitropic response rates of mesocotyl-coleoptiles in the ehd1 mutant. Error bars represent ses (n = 15). **P < 0.01 (Student’s t test) indicates a significant difference between the wild type and the ehd1 mutant. A lsd test was used to assess differences between the wild type and the ZmEHD1 KO mutant. C, The fluorescence of ZmDR5::mRFP in the wild type and the ehd1 mutant. D, Fluorescence of ZmPIN1a::YFP in the wild type and the ehd1 mutant. Scale bars = 50 μm. cc, Central cylinder; me, root meristem; rc, root cap; co, cortex. E, Free IAA content in wild-type maize and ehd1 mutant kernels at 15 DAP. Error bars represent ses (n = 4). **P < 0.01 indicates a significant difference from the wild type according to Student’s t test.