Table 1.
Surface-associated markers of seminal prostasomes from normozoospermic men separated by lectin-affinity chromatography.
| Column | LAC-separated fractions | sPro-N1a |
sPro-N2a |
||||
|---|---|---|---|---|---|---|---|
| GGT (U/g protein)b | ALP (U/g protein)b | CD63c | GGT (U/g protein)b | ALP (U/g protein)b | CD63c | ||
| Con A | Non-bound | 513 | 46 | +++ | 612.3 | 10.1 | +++ |
| Bound | 102.6 | 54.8 | + | 127.3 | 316.7 | +/− | |
| WGA | Non-bound | 294.4 | 31.6 | +++ | 371.7 | 6.8 | +++ |
| Bound | 242.4 | 2.8 | ++ | 208.2 | 0 | ++ | |
a
sPro-N1 and sPro-N2: different isolates of seminal prostasomes from normozoospermic men.
b
Enzyme activity (U/L) was normalised to concentration of proteins (g/L) in corresponding fraction.
c
CD63: immunoreactivity as determined by dot-blot (+++ intense; ± low/hardly visible).
ALP: alkaline phosphatase; GGT: gamma-glutamyl transferase.