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. 2020 Feb 24;9(1):439–456. doi: 10.1080/22221751.2020.1722758

Figure 2.

Figure 2.

Effects of SADS-CoV infection on caspase activation and PARP cleavage in vitro and in vivo. (A and B) SADS-CoV infection activates caspase-8, -9, -3 and cleaved PARP in vitro. Western blot analysis of caspase activation in SADS-CoV-infected cells (0.1 MOI) at different times or MOIs at 24 hpi. GAPDH was used as internal loading control. (C) Representative microphotographs of viral antigen immunochemical staining in SADS-CoV -non-infected and -infected ileal tissues (Bar: 200 μm). (D) SADS-CoV infection activates caspase-3 and cleaved PARP in vivo. Western blot analysis of the protein expression levels of caspase-3 and cleaved PARP in ileal samples from SADS-CoV -non-infected and -infected piglets at 24, 36, and 48 hpi. GAPDH was used as internal loading control.