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. 2020 Feb 24;27(1):323–333. doi: 10.1080/10717544.2020.1716882

Figure 2.

Figure 2.

Characterization and cellular uptake of PDMAEMA-NP and CPDMAEMA-NP. (A) Morphology of the developed nanocomplexes by TEM analysis after negatively stained with sodium phosphotungstate solution. (B) DLC evaluation of particle size distribution of PDMAEMA-NP and CPDMAEMA-NP. (C) Qualitative investigation of cellular uptake of PDMAEMA-NP and CPDMAEMA-NP in vitro by the fluorescence microscope. (D) Quantitative evaluation of cellular internalization of PDMAEMA-NP and CPDMAEMA-NP in vitro by the Flow cytometer. *p<.05, **p<.001, ***p<.001 vs. the cells treated by PDMAEMA-NP. (E) Investigation of the cellular uptake mechanism by pre-treating cells with various endocytosis inhibitors for 1 h. *p<.05 vs. the control group.