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. 2020 Jan 21;219(3):e201908142. doi: 10.1083/jcb.201908142

Video 4.

EGFP-Hsc70 fails to be recruited by clathrin-coated vesicles. Bottom surface of a gene-edited AP2-TagRFP+/+ SUM159 cell transiently expressing EGFP-Hsc70 imaged by TIRF microscopy every 1 s for 112 s. The time series shows diffuse cytosolic fluorescence signal and absence of dots corresponding to clathrin-coated vesicles.