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. 2020 Feb 18;219(3):e201906130. doi: 10.1083/jcb.201906130

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Figure S9. — PM and ER localization of the BcPI-PLC^H82A probe in response to endogenous PLC activation. (A–C) A summary schematic of the experimental design is presented alongside the kinetics of BcPI-PLC^H82A (B and C; PM-H82A^BRET), PI4P (C; PM-PI4P^BRET), and PI(4,5)P₂ (C; PM-PI(4,5)P₂^BRET) levels within the PM after treatment with AngII (100 nM). Please note that the green BRET trace shown in B has been expanded in C to scale the magnitude of the changes observed. (D) In addition to the PM, the levels of BcPI-PLC^H82A (ER-H82A^BRET) within the ER are also shown after AngII stimulation (100 nM). BRET measurements are presented as mean values ± SEM from three independent experiments performed using triplicate wells.