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. 2020 Feb 24;219(3):e201906031. doi: 10.1083/jcb.201906031

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© 2020 Vishnoi et al.

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Figure 6. — Nup188 directly interacts with Cep152. (A) Anti-GFP nanobody immunoprecipitations (IPs) were performed on cell extracts derived from HEK293T cells coexpressing FLAG-Nup188 and either GFP, GFP-Cep192, GFP-Cep152, or GFP-PCM1. Western blots using the indicated primary antibodies were performed to detect proteins in input (2%) and IP (100%) fractions. (B) ³⁵S-labeled Nup188 was generated in a reticulocyte vitro transcription/translation mix (IVT; left lane) and used to probe a nitrocellulose membrane with the indicated immobilized proteins generated from IPs (see Ponceau panel for total protein loads). Specific binding of ³⁵S-Nup188 was detected by autoradiography (AR). Numbers at left of all panels are position of molecular weight markers (in kilodaltons).