Fig. 4.

Role of mitophagy in the protective effect of PD against kidney injury in SI-AKI. The mice were subjected to CLP and administered vehicle, PD (30 mg/kg), or PD plus mdivi-1 (3 mg/kg), which is an inhibitor of mitophagy. Parkin−/− mice were subjected to CLP and treated with PD (30 mg/kg). The mice were sacrificed at 12 h following CLP. a H&E staining of the renal cells was performed for histological analysis (×400). Scale bar: 500 μm. b The tubular injury score was calculated based on visible damage to renal tubule cells. c Kim-1 expression was assessed by western blot analysis. Quantification of d Kim-1, e serum creatinine, f serum TNF-α, g serum IL-1β, and h serum IL-6