Table 2.
Number of CD1b+, S100+, MHC-II+ and CD83+ cells in HLNs (medullary, paracortical and follicular areas) and livers expressed as the mean ± SD per area of 0.08 mm2per animal
| Antibody | Group | Medullary area HLNs | Paracortical area HLNs | Follicular area HLNs | Livers |
|---|---|---|---|---|---|
| CNA.42 | UC | – | – | 43.1 ± 11 | – |
| 9 dpi | – | – | 71.6 ± 6.3 | 6.5 ± 10.8 | |
| 18 dpi | – | – | 93.2c ± 10.1 | 6.8 ± 10.1 | |
| CD1b | UC | 6.2 ± 0.8 | – | – | |
| 9 dpi | 12.7 ± 2.2 | – | 2.1 ± 3.4 | ||
| 18 dpi | 19.6c ± 4.6 | – | 5.7b ± 3.6 | ||
| S100 | UC | 8.7 ± 2.6 | 9.0 ± 6.9 | 14.9 ± 10.1 | 1 ± 0.1 |
| 9 dpi | 4.7 ± 0.9 | 5.8 ± 2.0 | 6.8 ± 4.3 | 2.3 ± 1.5 | |
| 18 dpi | 12.6b ± 1.5 | 16.3a ± 4.6 | 21.8a ± 7.1 | 3.9b ± 2.5 | |
| MHC-II | UC | 19.8 ± 1.7 | 34.2 ± 1.5 | 16.1 ± 1.7 | 11.8 ± 9 |
| 9 dpi | 13.4a ± 3.1 | 16.2a ± 3.8 | 1.6d ± 0.8 | 23.6b ± 5.5 | |
| 18 dpi | 1.9d ± 0.6 | 7.7d ± 4.2 | 0.2b ± 0.7 | 34d ± 9 | |
| CD83 | UC | 26.8 ± 6.4 | 53.1 ± 13.6 | 84 ± 10.4 | 0.6 ± 0.6 |
| 9 dpi | 67.4b ± 10.7 | 27a ± 5.4 | 29.7a ± 9.4 | 2 ± 2.9 | |
| 18 dpi | 75b ± 9.8 | 34.5 ± 1.4 | 18.9a ± 2 | 4.9c ± 2.5 | |
CNA.42 is expressed as the number of positive cells per germinal centre. Statistical differences are in comparison with the UC group.
UC: uninfected control group, dpi: days post-infection.
a(P < 0.05), b(P < 0.01), c(P < 0.001), d(P < 0.0001).