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. 2020 Mar 4;11:98. doi: 10.1186/s13287-020-01608-8

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© The Author(s) 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Characterization of hiPSCs and hiPSC-RPE. a Brightfield images of hiPSCs exhibiting typical colonies morphology. b, c hiPSCs expression of pluripotency markers OCT4 and SOX2. d, h Cell nucleus staining with DAPI. e Brightfield images of hiPSC-RPE cells display melanin pigmentation and polygonal morphology. f, g hiPSCs-RPE expression of typical RPE markers, cell boundaries when stained with ZO-1, and nuclear staining with MITF. Scale bar 200 μm (a, e) and 100 μm (b, c, d, f, g, h)