Fig. 4.

Survival of hiPSC-RPE and secretion of neurotrophic factors after transplantation into the rd10 mouse retina. a Schematic representation of hiPSC-RPE cell transplantation process into the subretinal space of rd10 mice. b Illustration of the hiPSC-RPE cells delivered into the potential space between retinal pigment epithelium and photoreceptors in the outer nuclear layer. c hiPSC-RPE cell survival analysis by HNA detected in transplanted rd10 retinal space day 7 post-surgery. d High magnification showed hiPSC-RPE cells were able to survive and integrate into host rd10 mouse retina by day 14 post-surgery. e The transplanted number of cells was 1000 ± 200, 800 ±100, and 700 ±100, at days 1, 7, and 14 post-surgery, respectively. f ELISA to quantify the concentration of PEDF in rd10 mice with and without transplantation. Scale bar 200 μm (c) and 50 μm (d). Data are presented as mean ± SEM. p values were determined by unpaired two-tailed Student’s t test (f), n = 4 for each group, *p < 0.05, **p < 0.01