Figure 7.

ACM containing Mt, but not Mt-depleted ACM, induced microglial HN synthesis. ACM or Mt-depleted ACM (mdACM, filtered ACM through a 0.22 μm pore-size column to remove Mt) was transferred onto cultured microglia. After 24 h, HNr mRNA levels (A) were assessed by SYBR real-time RT-PCR. Data are mean ± SEM; n = 5. One-way ANOVA/Fisher's LSD test: *p < 0.05 (p = 0.0380, Control vs ACM), t values (t = 2.358); *p < 0.01 (p = 0.0062, ACM vs mdACM), t values (t = 3.373). B, HNr protein levels were assessed by immune dot blot. Data are mean ± SEM; n = 3. One-way ANOVA/Fisher's LSD test: *p < 0.05 (p = 0.0366, Control vs ACM), t values (t = 2.678); *p < 0.05 (p = 0.0479, ACM vs mdACM), t values (t = 2.478).