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. 2020 Mar 4;40(10):2047–2055. doi: 10.1523/JNEUROSCI.2137-19.2019

Figure 5.

Figure 5.

Kv1.1 expression depends on age and experience. A, Examples of α-Kv1.1 staining in different rearing conditions. Images are single confocal slices (0.44 m) with identical laser, gain, and contrast settings for Kv1.1. Columns correspond to samples from different ages and rearing conditions. Top row, Kv1.1 signal in grayscale; bottom row, composite of Kv1.1 (cyan) with a fluorescent Nissl stain (magenta). Scale bar, 20 μm. B, Estimated proportion of Kv1.1-positive neurons at each age/condition tested. Compared with FR birds at P30–P35, the proportion of Kv1.1-positive neurons is higher in age-matched CR birds (GLMM: p = 0.001) and VI birds (p = 0.03). The proportion of Kv1.1-positive neurons is also greater in CR birds at P30–P35 than in CR birds at P18–P20 (p = 0.004). C, Higher-power images (63×) of Kv1.1 staining in cell bodies (top) and processes (bottom). Scale bars, 10 μm.