Table 1.

Mutant Library NGS Statistics: Summary of the of the Libraries Prior to Viral Genome Assembly

fragment	F1	F2	F3	G1	G2
residues	1–95	99–242	246–427	2–133	136–175
sequencing reads post quality filter	1 163 959	2 396 395	1 557 554	1 717 075	813 221
fold oversampling of codon combinations	441.1	792.5	412.1	619.4	821.9
percent of reads with
no nonsynonymous mutations	23.0%	33.1%	29.7%	33.3%	25.2%
one nonsynonymous mutation	52.6%	37.4%	35.5%	38.9%	59.7%
multiple nonsynonymous mutation	24.4%	29.4%	34.7%	27.7%	15.1%
coverage of possible single nonsynonymous mutations	100%	99.9%	100%	99.8%	100%