Table 2.
fragment | F1 | F2 | F3 | G1/G2 | WT |
---|---|---|---|---|---|
possible mutants | 1900 | 2880 | 3640 | 3440 | NA |
viable transformants | 2300 | 460 | 5.840 | 19 200 | 6400 |
plaques sequenced | 34 | 25 | 30 | 60 | 30 |
wildtype plaques | 17 (50%) | 11 (44%) | 15 (50%) | 30 (50%) | 30 (100%) |
Summary table of number of possible mutant viruses, number of viable viruses recovered from one transformation, and the number of mutant and wildtype genotypes recovered. No mutant genotypes were observed more than once.