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. Author manuscript; available in PMC: 2020 Nov 19.
Published in final edited form as: Acc Chem Res. 2019 Oct 8;52(11):3039–3050. doi: 10.1021/acs.accounts.9b00391

Figure 6.

Figure 6.

Benzimidazole luciferins exhibit unique patterns of light emission with Fluc and mutant luciferases. (A) Bioluminescent spectra of the analogs (blue) and D-luciferin (gray). (B) Unique patterns of light emission were observed. Mutants were arrayed across 96-well plates and treated with either the benzimidazole (top) or N-methylated (bottom) variant. Sample bioluminescence images are shown. Yellow and red boxes indicate enzymes that preferentially process the benzimidazole and N-methylated analogs, respectively. Part (A) was adapted with permission from ref. 35. Copyright 2012 American Chemical Society.