Supplementary Table I.
Location and sequences of primers used for extracting data on each hypervariable region (HVR) and combinations of adjacent HVRs
| HVR | Location ofthe forward primer | Sequence of the forward primer | Location of the reverse primer | Sequence of the reverse primer | Reference |
|---|---|---|---|---|---|
| V2 | 119 | AGYGGCGNACGGGTGAGTAA | 338 | TGCTGCCTCCCGTAGGAGT | 1 |
| V3 | 341 | CCTACGGGAGGCAGCAG | 534 | ATTACCGCGGCTGCTGG | 2 |
| V4 | 577 | AYTGGGYDTAAAGNG | 785 | TACNVGGGTATCTAATCC | 3,4 |
| V5 | 784 | AGGATTAGATACCCT | 907 | CCGTCAATTCCTTTGAGTTT | 5,6 |
| V6 | 961 | TCGAtGCAACGCGAAGAA | 1085 | ACATtTCACaACACGAGCTGACGA | 7 |
| V7 | 1099 | GYAACGAGCGCAACCC | 1238 | GTAGCRCGTGTGTMGCCC | 8,9 |
| V8 | 1050 | ATGGCTGTCGTCAGCT | 1385 | ACGGGCGGTGTGTAC | 10 |
| V2+V3 | 119 | AGYGGCGNACGGGTGAGTAA | 518 | ATTACCGCGGCTGCTGG | 1,2 |
| V3+V4 | 341 | CCTACGGGRSGCAGCAG | 798 | GGGGTATCTAATCCC | 2,11 |
| V4+V5 | 577 | AYTGGGYDTAAAGNG | 907 | CCGTCAATTYYTTTRAGTTT | 3,5,6 |
| V5+V6 | 805 | GACTACCAGGGTATCTAATCC | 1065 | AGGTGCTGCATGGCTGT | 9 |
| V6+V7 | 967 | CAACGCGAAGAACCTTACC | 1238 | GTAGCRCGTGTGTMGCCC | 9 |
| V7+V8 | 1046 | ACAGCCATGCAGCACCT | 1406 | GACGGGCGGTGWGTRCA | 9 |
N, any nucleotide; Y, C or T; D, not C; V, not T; R, A or G; M, A or C; lowercase nucleotide, low confidence base