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. 2020 Mar 4;10:4024. doi: 10.1038/s41598-020-60844-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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No significant modulation of proliferation upon hormonal treatment in AR-negative PCa cell lines (PC3 and DU145). Cell number of DU145 (A) and PC3 (B) cells after treatment with estrogenic and anti-estrogenic compounds detailed in the legend at time point 0 h, 48 h and 96 h. Values were determined with crystal violet staining and are presented as the average ± standard error of the mean (n = 8 samples/treatment). Data from one representative experiment out of three independent experiments is shown. There was no reproducible significant modulation of proliferation.