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. 2020 Mar 4;10:3986. doi: 10.1038/s41598-020-60747-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Venn diagrams of active chemicals for autofluorescence assays, (A) comparing different color channels, (B) considering only the blue channel and comparing cell lines/culture conditions, (C) considering only the green channel and comparing cell lines/culture conditions, and (D) considering only the red channel and comparing cell lines/culture conditions. For specific color channels cell culture conditions are represented as cell based (hepg2_cell and hek293_cell) and cell free, culture medium only (hepg2_free and hek293_free).