Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Mar 4;11:1178. doi: 10.1038/s41467-020-14981-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a Map of the donor plasmid pAcc-B with details of the carotenoid cassette (orange arrow). Red and blue arrows represent the homology arms. The two vertical green triangles mark the positions of the guide RNA B target sites. The nucleotide sequence of the donor plasmid is presented in Supplementary Data 1. Primers used to genotype the donor plasmid are marked on the map. b Map of the CRISPR plasmid pCam1300-CRISPR-B. Genes encoding Cas9p, gRNA-B, and hygromycin resistance (Hyg^R) are represented by purple, green, and black arrows, respectively. The Cas9p module is shown in detail. Primers used to genotype Cas9p are marked on the map. c, Scheme for transformation, selection, and regeneration.